NHS Fife’s Public Health Department is investigating a cluster of two confirmed cases and two suspected cases of E. coli O157 within the Dunfermline area.
The confirmed cases are recovering at home, as are the suspected cases.
Dr Margaret Hannah, NHS Fife Deputy Director of Public Health, said: “Although the source of the infection has not yet been confirmed we are following positive leads and have implemented control measures where applicable.
“We are working closely with our partners in Environmental Health, Health Protection Scotland and the Food Standards Agency to ensure that all appropriate safeguards are hygiene controls are in place.
“The incubation period for E. coli O157 is usually around one to 14 days. Anyone who is experiencing symptoms including diarrhoea, nausea, vomiting, bloody stools, stomach pain and fever should contact their GP or telephone NHS24 on 111.”