A major road into Freuchie was sealed off yesterday as an investigation was launched following reports of someone being injured on board a bus.
The mystery centred on a Moffat and Williamson bus going from St Andrews to Glenrothes.
It was still on the narrow B936, called East End, of the North East Fife village hours after the first reports emerged on Monday lunchtime.
Police were stationed at either end of the road, which leads from the A92 into the heart of Freuchie, allowing only locals through the cordon while investigations were carried out.
Traffic was being diverted through High Street as the bus remained in the middle of the residential street.
It is still unclear what caused the major disruption in the quiet village, but it was said locally that an elderly woman passenger had taken ill.
Villagers saw someone being taken from the scene on a stretcher and placed in an ambulance to be rushed to hospital.
The road was reopened by Tuesday morning.
A spokesman for the Scottish Ambulance Service said there had been a “medical issue”.
However, it was reported locally that a fellow passenger had had to perform CPR on the gravely ill woman.
The incident happened at around 12.15pm on the Moffat and Williamson 64 service, which runs from St Andrews to Glenrothes.
The bus was making its way along the narrow street, which is clogged with parked cars, when something happened.
One woman, who asked not to be named, said: “I heard that the woman was sitting on the long row of seats at the front, facing into the bus.
“People were saying the bus braked and she knocked into the pad which is there for wheelchair users.
“I believe a woman had to give her CPR to try to bring her round.”
She added rumours were the woman, who is thought to be in her 80s, was travelling on the bus with her granddaughter.
“I don’t think they are local to Freuchie.
“The bus was coming from St Andrews to Glenrothes, so it might have been they were from that area.”
Another local man passed by the incident shortly afterwards and spoke of seeing people, believed to be passengers, on the pavement, including one young woman who was visibly very upset.
Moffat and Williamson did not wish to comment while investigations are being carried out.