Bosses have been locked in discussions over whether to postpone or cancel the event after the Scottish Government announced a ban on gatherings of more than 500 people on Thursday.
Harry Styles, Dua Lipa, Calvin Harris and Biffy Clyro were among the acts due to play the Big Weekend in Dundee.
The BBC has now confirmed the Big Weekend will not be taking place this year, although talks are continuing over whether it could be held in Dundee at a later date.
A statement from BBC Radio 1 reads: “After careful consideration we have come to the decision that Radio 1’s Big Weekend 2020 in Dundee will not go ahead.
Part of huge crowd at the Radio 1 Big Weekend, Dundee, in 2006.
“This decision has been made in conjunction with our event partners, in line with the advice from the Scottish Government, and while we know fans looking forward to purchasing tickets will be disappointed, it is important that we prioritise the health and safety of all those involved.”
As of Friday afternoon there were 85 confirmed cases of coronavirus in Scotland.
There are now 798 cases across the UK, up from 590 on Thursday. Ten people across the country have died after contracting the disease.
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