Police are now treating the death of a Kirkcaldy man as murder.
Following the results of a post-mortem examination, officers confirmed that the death of Darren Adie has now become a murder inquiry.
The 42-year-old was found unconscious on Tweed Avenue, close to the junction with Lawson Street, around 6.45pm on Saturday.
He was taken to the Victoria Hospital where he later died.
Detective Chief Inspector Raymond Brown of the Police Scotland Major Investigation Team, said: “Our condolences go to Darren’s family and friends at this extremely difficult time.
“A thorough and robust investigation is currently underway to establish the full circumstances surrounding Darren’s death.
“I want to thank the community for their cooperation so far and urge anyone with information who has not yet come forward to get in touch.”
Local sources said the 42-year-old father of two was attacked before he collapsed in Tweed Avenue on Saturday evening.
It is believed he was stabbed before slumping to the ground.
Darren was a father of two young children and from the Kirkcaldy area.
Inspector Graeme Neill reassured local residents that such incidents were rare.
“I understand that any violent crime like this would cause a lot of anxiety for residents in the local area. That’s why we’re here to provide some reassurance,” he said.
“Anything like this can have a detrimental effect in the community but, looking at the bigger picture, it’s an isolated incident.”
Meanwhile, Mr Adie’s niece Tammy paid tribute to him on social media.
She said she had seen her uncle in the hour before he died.
“No words can explain how I feel,” she said. “What I would do to see him again.”
Mr Adie is believed to have originally been from the Dysart area.
Friend Shaun Aitchison said: “I’ve known him most of my life. He was always nice to me and was a good dad to his kids.”
One neighbour, who did not want to be named, said: “He was walking down from the lane and he just dropped. He had supposedly been stabbed.”