The incident happened just after 7pm on Thursday at Bogside.
Police in Fife are now appealing for witnesses following the fatal road collision on the A907.
The incident happened just west of the Devilla quarry.
The 31-year-old male motorcyclist sustained serious injuries when his bike was involved in a collision with a Ford Transit van.
Emergency services responded but sadly the man was subsequently pronounced dead.
Road policing officers closed the road for around five and a half hours while they carried out an investigation at the scene.
Anyone who can assist police with their ongoing inquiries is asked to come forward.
Sergeant Derek Allan from the Road Policing Unit in Glenrothes said: “Tragically, this collision has resulted in the motorcyclist sustaining fatal injuries and we are now continuing with our investigation to establish the full circumstances of what took place.
“Anyone who was on the A907 on Thursday evening and witnessed the incident is asked to contact police immediately.”
Those with information can contact Police Scotland on 101.
