The discovery took place at an address in Methil Brae in Methil after officers were given a tip-off from a member of the public.
They were able to recover 134 cannabis plants worth more than £30,000.
The property was empty when the police raided the cannabis cultivation and now the hunt is on to find the occupiers.
Detective Sergeant Jim McGuinness of Levenmouth CID said: ”We are committed to targeting those who deal in drugs with a view to preventing the harm they cause in our community.
“This was a significant recovery and it is essential that we trace the occupiers of this address as soon as possible.
“With this in mind, we will act on all intelligence we receive from members of the public and would encourage those with information to get in touch with us at their earliest convenience.”
