The Ministry of Defence is to be pressed for assurances about the future of Rosyth following the closure of HMS Caledonia.
Local MP Douglas Chapman fears the end of any naval presence in the town would have “significant repercussions” for the future.
Mr Chapman is now seeking a meeting with defence secretary Sir Michael Fallon to discuss issues including the future refitting of new aircraft carriers at Rosyth .
Fife Council leader David Ross has also asked for talks regarding the possibility of retaining a Royal Navy presence and potential future uses of the site.
Sir Michael announced last month HMS Caledonia and Condor Airfield in Angus would close, despite a £1.7 billion investment for the armed forces in Scotland.
The MoD wants to locate the military at “fewer, better locations”, a move which would see Leuchars Station become the army’s main hub north of the border.
While the Leuchars announcement has been welcomed, fears have been expressed for Rosyth once the navy moves out in 2022.
Mr Chapman said: “I have written to the Secretary of State for Defence asking for a meeting as the end of any naval presence in Rosyth has significant repercussions for the future.
“One would be the refitting of the new carriers at Rosyth – Caledonia is currently acting as a huge hotel for the crew of HMS Queen Elizabeth which will be going to sea very soon.
“If Caledonia closes then that accommodation facility will go too, which diminishes Rosyth’s opportunity to carry out carrier refits in the future.”
The MP said the west Fife town was the only place in the UK where carrier refits could be done and added: “Why give yourself a huge headache about where crew are stationed while each carrier is being refitted?”
Mr Ross said Fife Council had already requested a meeting with the MoD to discuss the expected increase in units and personnel numbers at Leuchars and to explore any requirements for services such as education and health locally.
“With HMS Caledonia in Rosyth identified as one of those bases scheduled for closure in 2022, we also want to discuss the possibilities for retention of a Royal Naval presence in Rosyth and potential future uses for the site,” he said.
Rosyth councillor Sharon Wilson has started discussions with the council’s economic development team regarding an overall development plan for the area.