Dundee attractions Discovery Point and Verdant Works have shut again just three months after reopening.
Dundee Heritage Trust made the decision to close immediately shortly after the Scottish Government rules Dundee is to remain in Tier 3.
Both venues had to close earlier in the year due to the UK wide lockdown and remained closed for four months.
An initial limited reopening on July 17 was followed by a full reopening on August 12.
Bosses have said they will review the closure at the beginning of December.
Deirdre Robertson, CEO of Dundee Heritage Trust said, “Sadly the impact on our visitor numbers from the Tier 3 designation for Dundee has been dramatic.
“Following reopening in July we have been overwhelmed by the support of the local community, and indeed those from further afield.
“In these uncertain times things change daily though and now, understandably, people are wary of venturing out and this combined with travel restrictions across the country has resulted in the very hard choice of temporary closing once again.”
The initial closure happened during what is traditionally the busiest months of the year for visitor attractions.
Verdant Works and Discovery Point were particularly badly hit by the closure due to the fact Dundee Heritage Trust is an independent charity which is over 98% self-funded.
The trust have now launched a brand new online shop selling bespoke and themed gifts.
Ms Robertson added: “These uncertain times really do impact on our hard working and dedicated staff and volunteers.
“I would urge anyone who would like to find out how to support the work we do to visit our websites, online shop or follow us on our social media platform’s and give what you can at this time to help us tell the incredible story of Dundee for now and for many generations to come.”