Childminding services in Perth and Kinross are said to be failing despite a multi-million-pound drive to expand provision for under-fives.
The Scottish Government has committed more than £969 million to deliver an expansion of early learning and childcare.
It has said childminders will play a key role in the delivery of the scheme, which will almost double the free entitlement to 1140 hours a year.
The Scottish Childminding Association (SCMA) has, however, warned that childminders are being largely excluded, with the benefit going to larger nurseries.
Within Perth and Kinross, that is thought to have placed many individuals who provide early years care in great difficulty.
Mid-Scotland and Fife Conservative MSP Alexander Stewart has raised their plight in the Scottish Parliament.
He is now awaiting answers to his questions, which include asking how ministers will ensure vital childminders are not overlooked.
The most recent Care Inspectorate figures show just 23% of local authority nurseries nationally can provide care for two-year-old children, compared to 92% of childminders.
Mr Stewart said: “There is a real crisis for childminders across Perth and Kinross.
“I have received multiple emails and phone calls from anxious and upset childminding constituents whose futures are in serious jeopardy and whose livelihoods are at risk as a result of this.
“These are incredibly difficult times for them and they are extremely worried.
“I have asked the Scottish Government what action it is taking to improve the accessibility of childminders for all families.
“I have also asked what action is being taken to recognise the vital role childminders play, and ensure they are closely integrated into the expansion of funded childcare hours.”
The Scottish Childminding Association believes that of the 6,000 or so childminders in Scotland, just 100 are currently commissioned by local authorities to deliver funded childcare.
The Scottish Government has said ministers recognise the contribution made by childminders, in relation to the needs of younger children in particular.
