Wildlife campaigners have said more should be done to prevent animals dying on the road.
The warning comes after a dead beaver was spotted in the central reservation of the A9, south of the Aberuthven turn off.
Badgers, otters, roe deer, hedgehogs and many other mammals are also often found dead at the roadside after being struck by a vehicle.
The roadkill collisions also cause danger, expense and inconvenience to thousands of motorists in rural areas every year.
Rosie Third, Environmental Action Killin, worked with engineers to install reflective devices to frighten wild animals from crossing busy stretches of road.
She was moved to act after discovering a dead otter by the side of the A827.
Engineers installed the devices – which use car headlights to dazzle animals and stop them running out of verges – near the Acharn biomass facility, near Killin, last year.
Rosie said they appeared to have worked.
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“There haven’t been any animals killed at that point of the road since we fitted the reflectors,” she said.
“There also hasn’t been any sightings of wildlife crossing the road at that point either.
“We don’t know for definite that it is the reflectors causing it, but we hope so.”
She said wildlife groups from Lancashire and other areas had contacted her since reading about the reflectors to ask about installing them in their area.
“When an animal runs out in front of a car it happens in a flash and there’s very little you can do about it. It is also very dangerous for the drivers so I would be very keen to see if the reflectors are truly effective in reducing these types of accidents.”
Motorist Graham McQuillan spotted the dead beaver in the central reservation of the A9.
“I first saw it heading north on A9 on Wednesday afternoon and saw it lying on its side and could see its big flat tail,” he said.
“I have since passed it another three times and it’s still there.”
A spokesperson for the Scottish Wildlife Trust said: “Beavers spend most of their time in the water but they will come on to land occasionally. Sadly, in common with all wildlife, beavers are vulnerable when they have to cross roads.
“We encourage people to report sighting of any mammals, including those killed on the road, through recording schemes like Mammal Tracker.
“This builds a better understanding of where different species can be found, and may also help identify hotspots for road deaths.”