Work is expected to start soon on the Dunfermline site for Amazon, with diggers already in place.
The online retail firm will create 763 jobs at the site at Calais Muir South, alongside the M90 near the junction with the A92.
JCB diggers are now in place as preparatory work is to begin at Sandpiper Drive.
The new centre in Dunfermline will be Amazon’s biggest in the UK, measuring almost the size of 14 football pitches.
The company will transfer around 100 staff from its site in Glenrothes to the Dunfermline office.
Employees in Dunfermline will distribute a range of items throughout the world and will also provide extra capacity for a wide selection of products.
A spokeswoman for Amazon confirmed work will begin shortly at the Dunfermline site, in a project that is estimated will cost around £60 million.
She said, “We look forward to work commencing on the Dunfermline site shortly and we expect the centre to be operational before the end of 2011. We will starting recruiting in the second half of 2011.
Amazon said it will create 763 jobs in Dunfermline, with the possibility of a further 200 subcontracting jobs and an additional 1500 jobs during busier periods, such as in the run-up to Christmas.
Planning permission had already been in existence for the Dunfermline site and came about after talks between Amazon, the Scottish Government, Fife Council, Scottish Enterprise and Scottish Development International.
Amazon will also create 200 posts at its site in Gourock on the west coast.